Resumo Objetivou-se analisar as características sociodemográficas das minorias sexuais e de gênero que convivem frequentemente com filhos(as) e verificar se existe associação entre convívio frequente com filhos(as) e os cuidados em saúde. Trata-se de um estudo transversal com dados do inquérito de saúde LGBT+, realizado no Brasil em 2020 (agosto-novembro) de forma on-line e anônima, totalizado 958 participantes. O convívio frequente com filhos(as) foi avaliado pela moradia com filhos(as) ou encontros presenciais quinzenais com filhos(as) que moram em outro domicílio. Os cuidados em saúde incluíram ter um profissional ou serviço de referência, sentir-se à vontade para contar seus problemas e receber tratamento médico ou hospitalar de pior qualidade. A regressão de Poisson com variância robusta foi usada na análise estatística. A prevalência de convívio com filhos(as) foi de 5,3%. Após o ajuste por idade, verificou-se uma prevalência estatisticamente maior em mulheres cisgênero (13,4%) e entre pretos/pardos e outras raças/cores não brancas (7,9%). Observou-se que o convívio frequente com filhos(as) foi positivamente associado apenas a receber tratamento médico ou hospitalar de pior qualidade (RP=6,00; IC95% 1,22-29,67). Esses achados destacam que ainda há estigma/preconceito nos serviços de saúde.

Abstract This study aimed to examine the sociodemographic profile of sexual and gender minorities who regularly interact with children and investigate whether such frequent interactions are associated with healthcare factors. This cross-sectional study utilized data from the LGBT+ Health Survey in Brazil, conducted online and anonymously from August to November 2020 with 958 participants. Regular interaction with children was defined as living with children or engaging in bi-weekly face-to-face meetings with children residing in different households. Healthcare factors encompass having a professional or reference service, feeling comfortable in discussing personal issues, and receiving worse quality medical or hospital care. The statistical analysis used the Poisson regression with robust variance. The prevalence of interaction with children was 5.3%. We observed a statistically higher prevalence among cisgender women (13.4%) and Black/brown and other non-white people (7.9%) after adjusting for age. The results showed a positive association only between regular interaction with children and worse-quality medical or hospital care received (PR=6.00; 95%CI 1.22-29.67). These findings highlight a persistent stigma and prejudice within healthcare services.

ABSTRACT Background: This manuscript provides an overview of liver carcinogenesis in murine models of hepatocellular carcinoma (HCC) and cholangiocarcinoma (CCA). Objective: A review through MEDLINE and EMBASE was performed to assess articles until August 2022. Methods: Search was conducted of the entire electronic databases and the keywords used was HCC, CCA, carcinogenesis, animal models and liver. Articles exclusion was based on the lack of close relation to the subject. Carcinogenesis models of HCC include HCC induced by senescence in transgenic animals, HCC diet-induced, HCC induced by chemotoxicagents, xenograft, oncogenes, and HCC in transgenic animals inoculated with B and C virus. The models of CCA include the use of dimethylnitrosamine (DMN), diethylnitrosamine (DEN), thioacetamide (TAA), and carbon tetrachloride (CCl4). CCA murine models may also be induced by: CCA cells, genetic manipulation, Smad4, PTEN and p53 knockout, xenograft, and DEN-left median bile duct ligation. Results: In this review, we described different murine models of carcinogenesis that reproduce the key points for HCC and CCA genesis allowing a better understanding of its genetic, physiopathological, and environmental abnormalities. Conclusion: Each model has its advantages, disadvantages, similarities, and differences with the corresponding human disease and should be chosen according to the specificity of the study. Ultimately, those models can also be used for testing new anticancer therapeutic approaches.

RESUMO Contexto: Este manuscrito fornece uma visão geral da carcinogênese hepática em modelos murinos de carcinoma hepatocelular (CHC) e colangiocarcinoma (CCA). Objetivo: Realizar uma revisão de artigos científicos até agosto de 2022 utilizando as bases de dados MEDLINE e EMBASE. Métodos: A busca foi realizada em todas as bases de dados eletrônicas e as palavras-chave usadas foram CHC, CCA, carcinogenesis, modelos animais e fígado. A exclusão dos artigos baseou-se na falta de estreita relação com o assunto. Os modelos de carcinogênese do CHC incluíram: CHC induzido por senescência em animais transgênicos, CHC induzido por dieta, CHC induzido por agentes quimiotóxicos, xenoenxerto, oncogenes e CHC em animais transgênicos inoculados com vírus B e C. Os modelos de CCA incluíram: o uso de dimetilnitrosamina (DMN), dietilnitrosamina (DEN), tioacetamida (TAA) e tetracloreto de carbono (CCl4). Os modelos murinos de CCA induzidos por incluir: células de CCA, manipulação genética, animais nocaute para Smad4, PTEN e p53, xenoenxerto e ligadura do ducto biliar mediano esquerdo. Resultados: Nesta revisão, descrevemos diferentes modelos murinos de carcinogênese que reproduzem os pontos-chave para a gênese do CHC e do CCA, permitindo uma melhor compreensão de suas anormalidades genéticas, fisiopatológicas e ambientais. Conclusão: Cada modelo tem suas vantagens, desvantagens, semelhanças e diferenças com a doença humana correspondente e deve ser escolhido de acordo com a especificidade do estudo. Em última análise, esses modelos também podem ser utilizados para testar novas abordagens terapêuticas anticancerígenas.

ABSTRACT Background: Recent studies show an increase in nonalcoholic fatty liver disease (NAFLD) in populations with higher consumption of red meat, processed and cooked at high temperatures. On the other hand, the single nucleotide polymorphism rs738409 in the Patatin-like phospholipase domain containing 3 (PNPLA3) gene has been implicated in susceptibility to NAFLD and liver fibrosis. However, the synergistic effect between red meat consumption and the PNPLA3 gene polymorphism in NAFLD has not yet been evaluated. Objective: To evaluate the association between the presence of the polymorphism in the PNPLA3 gene and the consumption of macronutrients, including meat consumption and its cooking method among NAFLD patients. Methods: This was a cross-sectional study with 91 patients diagnosed with NAFLD by liver biopsy with genotyping for the polymorphism in the PNPLA3 gene were included. The consumption of calories and macronutrients was verified using the semi-quantitative food frequency questionnaire and the specific questionnaire on meat consumption. PNPLA3 gene polymorphism was analyzed by real-time polymerase chain reaction (RT-PCR) and anthropometric evaluation was realized. Results: The mean BMI was 32.38±4.58 kg/m² and the waist circumference was 107±10 cm. On liver biopsy, 42% of patients had significant fibrosis (F≥2). The odds ratio of F≥2 was 2.12 for the GG group and 1.54 for the CG group, compared to the CC group. The mean caloric intake was 1170±463.20 kcal/d. The odds ratio in the CC group concerning high red meat consumption in comparison to low consumption was 1.33. For white meat, the odds ratio was 0.8 when comparing high and low intake, also in the CC group. Conclusion: High red meat intake and PNPLA3 gene polymorphism seem to synergistically affect NAFLD and liver fibrosis, requiring confirmation in a larger number of patients and in different populations.

RESUMO Contexto: Estudos recentes mostram um aumento da doença hepática gordurosa não alcoólica (DHGNA) em populações com maior consumo de carne vermelha, processada e cozida em altas temperaturas. Por outro lado, o polimorfismo rs738409 no gene Patatin-like fosfolipase contendo 3 (PNPLA3) tem sido implicado na suscetibilidade à DHGNA e fibrose hepática. No entanto, o efeito sinérgico entre o consumo de carne vermelha e o polimorfismo no gene PNPLA3 na DHGNA ainda não foi avaliado. Objetivo: Avaliar a associação entre a presença do polimorfismo no gene PNPLA3 e o consumo de macronutrientes, incluindo o consumo de carne e seu modo de cozimento em pacientes com DHGNA. Métodos: Realizamos um estudo transversal com 91 pacientes diagnosticados com DHGNA por biópsia hepática e genotipados para o polimorfismo no gene PNPLA3. O consumo de calorias e macronutrientes foi verificado por meio do questionário de frequência alimentar semi-quantitativo (QFA) e do questionário específico sobre consumo de carnes. O polimorfismo no gene PNPLA3 foi analisado por reação em cadeia da polimerase em tempo real (RT-PCR) e a avaliação antropométrica foi realizada. Resultados: O índice de massa corporal médio foi de 32,38±4,58 kg/m² e a circunferência da cintura foi de 107±10 cm. Na biópsia hepática, 42% dos pacientes apresentavam fibrose significativa (F≥2). O odds ratio de F≥2 foi de 2,12 para o grupo GG e 1,54 para o grupo GC, comparado ao grupo CC. A ingestão calórica média foi de 1.170±463,20 kcal/d. O odds ratio para alto consumo de carne vermelha no grupo CC em comparação ao baixo consumo foi de 1,33. Para a carne branca, este valor foi de 0,8 ao comparar o alto e o baixo consumo, também no grupo CC. Conclusão: A alta ingestão de carne vermelha e o polimorfismo no gene PNPLA3 parecem afetar sinergicamente a DHGNA e a fibrose hepática, necessitando de confirmação em maior número de pacientes e em diferentes populações.

Resumo O objetivo deste artigo é analisar a implantação do Programa de Promoção da Alimentação Adequada e Saudável na Atenção Primária à Saúde em municípios de Minas Gerais. Pesquisa de Métodos Mistos com Estratégia Incorporada Concomitante de dados. Na abordagem quantitativa utilizou-se pesquisa avaliativa na perspectiva da análise de implantação. Na abordagem qualitativa realizou-se estudo exploratório-descritivo utilizando a análise de conteúdo. Os dados qualitativos e quantitativos foram incorporados para análise a partir de sua imbricação. O Grau de Implantação do programa foi 92,5% representando uma implantação adequada. Na imbricação dos dados, a metodologia das ações propostas no programa e a realização da atividade de Educação Permanente foram pontos fortes convergentes que emergiram. Recursos Humanos insuficientes para planejamento e execução do programa e falta de cozinha para oficina culinária foram apontados como desafios. A implantação do programa foi adequada e poderá ser estendida para outros municípios brasileiros, de forma a apoiar os profissionais a estruturar intervenções de promoção da alimentação adequada e saudável na rotina de trabalho na Atenção Primária.

Abstract This article aims to analyze the implementation of an Adequate and Healthy Food Promotion Program in Primary Health Care in the municipalities of Minas Gerais. Mixed Methods Research with Data Concomitant Embedded Strategy. Evaluative research from the perspective of the implementation analysis was employed in the quantitative approach. An exploratory-descriptive study was conducted using content analysis in the qualitative approach. Qualitative and quantitative data were incorporated for analysis from their imbrication. The Program Implementation Level (IL) was 92.5%, representing an adequate implementation. The methodology of the actions proposed in the program and the implementation of Continuing Education were convergent strengths that emerged from data imbrication. The need for more human resources for planning and implementing the program and the lack of a kitchen for cooking workshops were identified as challenges. The Program’s implementation was adequate and can be extended to other Brazilian municipalities to help professionals structure interventions to promote adequate and healthy food in the Primary Care work routine.

Abstract This article aims to analyze the implementation of an Adequate and Healthy Food Promotion Program in Primary Health Care in the municipalities of Minas Gerais. Mixed Methods Research with Data Concomitant Embedded Strategy. Evaluative research from the perspective of the implementation analysis was employed in the quantitative approach. An exploratory-descriptive study was conducted using content analysis in the qualitative approach. Qualitative and quantitative data were incorporated for analysis from their imbrication. The Program Implementation Level (IL) was 92.5%, representing an adequate implementation. The methodology of the actions proposed in the program and the implementation of Continuing Education were convergent strengths that emerged from data imbrication. The need for more human resources for planning and implementing the program and the lack of a kitchen for cooking workshops were identified as challenges. The Program’s implementation was adequate and can be extended to other Brazilian municipalities to help professionals structure interventions to promote adequate and healthy food in the Primary Care work routine.

Resumo O objetivo deste artigo é analisar a implantação do Programa de Promoção da Alimentação Adequada e Saudável na Atenção Primária à Saúde em municípios de Minas Gerais. Pesquisa de Métodos Mistos com Estratégia Incorporada Concomitante de dados. Na abordagem quantitativa utilizou-se pesquisa avaliativa na perspectiva da análise de implantação. Na abordagem qualitativa realizou-se estudo exploratório-descritivo utilizando a análise de conteúdo. Os dados qualitativos e quantitativos foram incorporados para análise a partir de sua imbricação. O Grau de Implantação do programa foi 92,5% representando uma implantação adequada. Na imbricação dos dados, a metodologia das ações propostas no programa e a realização da atividade de Educação Permanente foram pontos fortes convergentes que emergiram. Recursos Humanos insuficientes para planejamento e execução do programa e falta de cozinha para oficina culinária foram apontados como desafios. A implantação do programa foi adequada e poderá ser estendida para outros municípios brasileiros, de forma a apoiar os profissionais a estruturar intervenções de promoção da alimentação adequada e saudável na rotina de trabalho na Atenção Primária.

ABSTRACT Introduction: Metabolic dysfunction-associated steatotic liver disease (MASLD), previously known as Nonalcoholic fatty liver disease (NAFLD), is one of the most common hepatic diseases in individuals with overweight or obesity. In this context, a panel of experts from three medical societies was organized to develop an evidence-based guideline on the screening, diagnosis, treatment, and follow-up of MASLD. Material and methods: A MEDLINE search was performed to identify randomized clinical trials, meta-analyses, cohort studies, observational studies, and other relevant studies on NAFLD. In the absence of studies on a certain topic or when the quality of the study was not adequate, the opinion of experts was adopted. Classes of Recommendation and Levels of Evidence were determined using prespecified criteria. Results: Based on the literature review, 48 specific recommendations were elaborated, including 11 on screening and diagnosis, 9 on follow-up, 14 on nonpharmacologic treatment, and 14 on pharmacologic and surgical treatment. Conclusions: A literature search allowed the development of evidence-based guidelines on the screening, diagnosis, treatment, and follow-up of MASLD in individuals with overweight or obesity.

ABSTRACT Background: Chagas disease (ChD) is a neglected tropical disease that is caused by the protozoan parasite Trypanosoma cruzi and can negatively impact quality of life (QoL). This study aimed to assess and compare QoL between individuals with and without ChD. Methods: This cross-sectional study was performed within a concurrent cohort study (REDS). The participants were derived from two blood donation centers: São Paulo capital and Montes Claros, Minas Gerais, Brazil. Participants with ChD were identified in blood donations by serological diagnosis between 2008 and 2010, and those without ChD were donors with negative serology identified during the same period. QoL was assessed using the World Health Organization Quality of Life-BREF questionnaire. Logistic regression was used to compare sociodemographic and clinical characteristics between the groups, and mean, standard deviation, and beta regression were used to compare QoL. Results: In total, 611 individuals participated in the study (328 with ChD and 283 without ChD). Participants with ChD had lower QoL in the physical (p=0.02) and psychological (p<0.01) domains than did individuals without CD. Conclusions: Individuals with ChD had worse QoL perceptions. These results provide a comprehensive understanding of the impact of ChD on individuals' QoL, while also highlighting potential opportunities for improving the care and treatment of those affected.

ABSTRACT Difficulties in executive functions and motor performance in children with autism spectrum disorder (ASD) have been researched. This study aimed to verify the effect of exergames on motor performance and reaction time of a child (6 years old) with ASD. Eight weeks of games with Xbox Kinect were applied, with weekly meetings and duration of 35 to 40 minutes. Motor performance was assessed by the Motor Development Scale (EDM) and reaction time by the Simple Reaction and Choice RT/S1 test, available in the computerized Mental Test and Training System. The data obtained were analyzed according to the JT Method. The results indicated a reliable positive change in the general motor quotient (QMGpre= 87.5/QMGpost= 102.32) with a reliable change index (IMC) of 4.19, in the general motor age (IMGpre= 70/IMGpost= 88; IMC= 4.55) and in the motor areas balance (QMpre= 52.5/QMpost= 104.65; IMC= 2.47) and body schema (IMpre= 60/IMpost= 96; IMC= 2.63) after application of the exergames, but without significant changes in the reaction time scores (TRpre= 361/TRpost= 319; IMC= 1.26). The findings suggest that the use of exergames seems to reflect improvements in motor aspects, but not in reaction time.

RESUMO Dificuldades nas funções executivas e no desempenho motor em crianças com o transtorno do espectro autista (TEA) têm sido alvos de pesquisas. Este estudo teve por objetivo verificar o efeito do exergames no desempenho motor e no tempo de reação de uma criança (6 anos) com TEA. Foram aplicadas oito semanas de jogos com o Xbox Kinect, com encontros semanais e duração de 35 a 40 minutos. O desempenho motor foi avaliado pela Escala de Desenvolvimento Motor (EDM) e o tempo de reação pelo teste Reação Simples e de Escolha RT/S1, disponível no sistema computadorizado Mental Test and Training System. Os dados obtidos foram analisados segundo o Método JT. Os resultados indicaram uma mudança positiva confiável no quociente motor geral (QMGpré= 87.5/QMGpós= 102.32) com um índice de mudança positiva confiável (IMC) de 4.19, na idade motora geral (IMGpré= 70/IMGpós= 88; IMC= 4.55) e nas áreas motoras equilíbrio (QMpré= 52,5/QMpós= 104,65; IMC= 2,47) e esquema corporal (IMpré= 60/IMpós= 96; IMC= 2.63) após aplicação do exergames, entretanto, sem alterações significativas nos escores do tempo de reação (TRpré= 361/TRpós= 319; IMC= 1,26). As descobertas sugerem que o uso do exergames parece refletir em melhorias nos aspectos motores, mas não no tempo de reação.

Abstract Background: Due to the population’s aging, the prevalence of chronic illnesses, including diabetes mellitus, is increasing, and nurses have to face the challenges arising from the complexity of older adults’ medication regimens and their optimal management. Objective: To understand how older adults with diabetes mellitus manage their medication regimens. Methodology: This is a descriptive qualitative study using an inductive approach. Twelve semi-structured interviews were conducted with older adults diagnosed with diabetes mellitus. The interviews were transcribed and analyzed using content analysis. Results: Therapeutic success relates to how patients deal with the need to adhere to a medication regimen. Despite patients recognizing the need to take medication, intrinsic factors (such as gender and age) and extrinsic factors (treatment duration, underdeveloped health systems, and others) influence therapeutic success. Conclusion: The study allowed understanding how patients manage their medication regimens. It confirmed that low adherence rates compromised therapeutic success and identified the urgent need for interventions that address the specific needs of chronically-ill patients.

Resumen Marco contextual: Ante el envejecimiento de la población, aumenta la prevalencia de enfermedades crónicas, entre ellas la diabetes mellitus. La complejidad de los regímenes de medicación de estas personas y la optimización de su gestión es un reto para los enfermeros. Objetivo: Entender cómo el anciano con diabetes mellitus gestiona su régimen de medicación. Metodología: Estudio descriptivo, cualitativo e inductivo. Se realizaron 12 entrevistas semiestructuradas a ancianos diagnosticados de diabetes mellitus. El texto se transcribió y analizó mediante un análisis de contenido. Resultados: El éxito terapéutico está relacionado con la forma en que las personas afrontan la necesidad de cumplir un régimen de medicación. Los factores intrínsecos (como el sexo y la edad) y extrínsecos (como la duración del tratamiento, el sistema sanitario poco desarrollado, entre otros) condicionan el éxito terapéutico, aunque las personas reconozcan como necesario tomar medicamentos. Conclusión: Este estudio permitió comprender cómo las personas gestionan su régimen de medicación, corrobora que las bajas tasas de adherencia comprometen el éxito terapéutico y establece la urgencia de llevar a cabo intervenciones dirigidas a las necesidades concretas de las personas con enfermedades crónicas.

Resumo Enquadramento: Face ao envelhecimento populacional existe uma crescente prevalência de doenças crónicas entre elas a diabetes mellitus. A complexidade dos regimes medicamentosos destas pessoas e a otimização da sua gestão assume-se desafiante para os enfermeiros. Objetivo: Compreender como a pessoa idosa com diabetes mellitus gere o seu regime medicamentoso. Metodologia: Estudo descritivo, qualitativo e natureza indutiva. Realizadas 12 entrevistas semiestruturadas a pessoas idosas com diagnóstico de diabetes mellitus. O verbatim foi transcrito e analisado através de análise de conteúdo. Resultados: O sucesso terapêutico relaciona-se com o modo como as pessoas lidam com a necessidade de aderir a um regime medicamentoso. Fatores intrínsecos (como sexo e idade) e fatores extrínsecos (como duração do tratamento, sistema de saúde pouco desenvolvido, entre outros) condicionam o sucesso terapêutico, ainda que a toma dos medicamentos seja reconhecida como necessária. Conclusão: Este estudo permitiu compreender como as pessoas gerem o regime medicamentoso, corrobora que baixas taxas de adesão comprometem o sucesso terapêutico e estabelece como prementes intervenções dirigidas às necessidades concretas das pessoas com doença crónica.

ABSTRACT Resistance training (RT) is a widely practised type of training, and the number of adherents of this type of physical exercise is increasing each year. Among the most sought objectives of those who initiate RT is muscle hypertrophy, although success in this process depends on a well-designed protocol and good manipulation of training variables. The present study aims to analyse, through a systematic review, the impact of the RT variables (such as intensity, volume, recovery interval, execution speed, and concentric muscle failure) relevant to muscle hypertrophy and if there is an ideal range for each training variable. The research was carried out in the PubMed, Web of Science, Scopus and Scielo databases from 2000 to 2020, using the terms “resistance training” and “hypertrophy” and “intensity” or “volume” or “recovery interval” or “execution speed” or “muscular failure”. Twenty-three articles were included in the review. The PEDro scale was used to analyse the quality of the selected articles. It was concluded that the variables intensity and volume must be carefully analysed in a training program. Despite not having a direct impact on hypertrophy, the other variables affect the intensity and volume and must be manipulated according to what is intended with the others.

ABSTRACT This work aimed to systematically review the literature to assess the effect of stretching on muscle strength in healthy people. The PubMed, Scopus and Scielo data sources were consulted, using the eligibility criteria: intervention with stretching and measurement of muscle strength performance in healthy people. Eighteen studies that met the criteria were included. The types of stretching used were continuous static 20%, intermittent static 60% and dynamic 20%. The types of strength evaluated were: maximum isometric strength (12.5%), maximum dynamic strength (66.66%) and muscle power (20.83%). 5.5% of the studies demonstrated increased muscle strength after stretching; 38.8% showed reduction after static stretching; 38.8% did not observe changes; and 16.6% had increased or reduced muscle strength depending on the type of stretching used. Based on the studies analyzed, performing static stretching prior to training, various manifestations of strength tended to reduce or not change muscle performance. It was not found a clear sign that the stretching performed before the strength training promotes an improvement in muscle performance.

RESUMO O presente trabalho teve por objetivo revisar sistematicamente a literatura, para avaliar o efeito do alongamento sobre a força muscular de pessoas saudáveis. Foram consultadas as fontes de dados: PubMed, Scopus e Scielo, utilizando-se os critérios de elegibilidade: intervenção com alongamento e medida de desempenho da força muscular, em pessoas saudáveis. Foram incluídos 18 estudos, que atenderam os critérios. Os tipos de alongamento utilizados foram o estático contínuo 20%, estático intermitente 60% e o dinâmico 20%. Os tipos de força avaliados foram: força máxima isométrica (12,5%), força máxima dinâmica (66,66%) e potência muscular (20,83%). 5,5% dos estudos demonstraram aumento da força muscular após o alongamento; 38,8% apresentaram redução, após o alongamento estático; 38,8% não observaram alteração; e 16,6% apresentaram aumento ou redução da força muscular dependendo do tipo de alongamento utilizado. Com base nos trabalhos analisados à realização de alongamento estático de forma prévia ao treinamento de diversas manifestações de força tenderam a reduzir ou não alterar o desempenho muscular. Não foi encontrada uma sinalização clara que o alongamento realizado antes do treino de força promova uma melhora de rendimento muscular.

ABSTRACT Introduction: The oral cavity can present the first clinical manifestations of leukemia, therefore; it is important to recognize their principal characteristics. Objective: To identify oral manifestations as the first clinical signs of leukemia. Methods: This is an integrative review, that gathered data from articles with oral manifestations of leukemia as part of its first clinical features. The were included case reports, case series, clinical research, or reviews with case reports. The variables that were considered relevant: age, sex, sites of the oral lesions, characteristics of the oral lesions, medical history and physical examination, time of evolution, radiographic examination, blood test results, initial diagnosis, differential diagnosis and final diagnosis. Results: A total of 31 studies were included, with a total of 33 individuals identified. There were 19 (57.57%) males and 14 (42.42%) females. The age range was from 1.6 to 74 years. Acute myeloid leukemia (72.72%) and acute lymphoid leukemia (18.18%) presented more oral manifestations as the first clinical signs of the disease. All individuals with leukemia presented lesions, such as ulcer, erosion, bleeding, ecchymosis, color change of the bluish or pale mucous membranes and areas of tissue necrosis. Hard tissue lesions were less frequent, being 6 (18.18%). Conclusion: The first clinical manifestations of leukemia can be present in the oral cavity, mainly in acute myeloid leukemia. The principal oral tissues affected were gingival tissue, buccal mucosa and hard and/or soft palate. When hard tissues, such as the maxilla bone or mandible bone were affected, dental mobility was the principal clinical sign.

Abstract Background Amaranthus spp. are problematic weeds and competitors for nutrients in several crops, especially in soybean and corn. Resistance to glyphosate has been detected in several weed species, and a triple mutation in its EPSPS target gene was detected recently in Amaranthus hybridus. Objective The aim of this work was to develop a simple polymerase chain reaction (PCR) method to detect the EPSPS triple mutation in A. hybridus. Methods Two pairs of primers were designed for PCR-based detection of the EPSPS TAP-IVS triple mutation, which confers resistance to glyphosate, in A. hybridus. Results These sets of allele-specific primers were tested on five Amaranthus species and in 65 different field accessions. The PCR reaction using one set of the primers amplifies the wildtype (TAP) allele while the PCR reaction using the other pair of primers amplifies the triple mutation (IVS) allele. The presence of PCR products in both sets of primers identifies the heterozygous resistant individuals, and PCR product amplified only with the triple mutation set of primers identifies the homozygous resistant individuals. A DNA concentration test was performed and the recommend DNA amount to be used is 100 ng. Conclusions We developed and tested two sets of primers to detect the EPSPS TAP-IVS triple mutation and the results showed a 100% genotypic to phenotypic association. The triple mutation detection assay is easy to use and can be applied in a molecular laboratory with basic equipments. Early detection of resistance helps to better manage and control its spreading.

RESUMO A doença linfoproliferativa pós-transplante (DLPT) é formada por um grupo heterogêneo de proliferações hematolinfóides resultantes da proliferação mono ou policlonal das células linfoides. O quadro clínico é variado e dependente dos sítios envolvidos, sendo o trato gastrintestinal e o sistema nervoso central os mais comuns, e sintomas constitucionais são frequentes. O envolvimento isolado do enxerto é raro. Relatamos aqui um caso de DLPT policlonal isolada do enxerto em um receptor de transplante renal com doador vivo HLA idêntico, ocorrido sete anos após o transplante. Digno de nota, o paciente não apresentou sintomas constitucionais e sua única manifestação clínica foi disfunção do enxerto, expressa através da elevação da creatinina e discreta proteinuria, sendo o diagnóstico realizado através de biópsia renal, que evidenciou infiltrado intersticial linfoide denso. Tratava-se de DLPT policlonal não relacionada ao vírus Epstein-Bar (EBV) e foi tratado com sucesso com quimioterapia, redução da imunossupressão e sirolimo.

ABSTRACT Post-transplantation lymphoproliferative disease (PTLD) comprises a heterogeneous group of hematolymphoid proliferations resulting from a monoclonal or polyclonal proliferation of lymphoid cells. The clinical presentation varies according to the affected sites. The gastrointestinal tract and the central nervous system are the most common, and constitutional symptoms are frequent. Isolated allograft involvement is rare. We report a case of polyclonal PTLD isolated in the kidney allograft in a patient who received an HLA-identical living donor seven years before. Noteworthy, this patient did not present constitutional symptoms, and his only clinical manifestation was graft dysfunction, expressed by an increase in serum creatinine and mild proteinuria. The diagnosis was performed through renal biopsy, which showed dense lymphoid interstitial infiltrate. The PTLD was polyclonal, unrelated to Epstein-Bar virus (EBV), and it was successfully treated with chemotherapy, reduced immunosuppression, and sirolimus.

RESUMO As lesões provocadas por projetil de arma de fogo em região de cabeça e pescoço implicam, geralmente, em morbidade e mortalidades severas representando um importante problema de saúde pública. Esses ferimentos podem ser classificados como: penetrantes, perfurantes e avulsivos, sendo este último o mais preocupante, por serem ocasionados por projéteis de alta velocidade. Os projéteis de baixa velocidade causam comumente ferimentos penetrantes, permanecendo por vezes alojados nos tecidos, podendo ou não, ser indicada a sua remoção, a depender de fatores como localização e gravidade da lesão. O presente artigo traz o relato de três casos clínicos que abordam lesões por projeteis de arma de fogo de baixa velocidade, nas quais tiveram a indicação de remoção do referido artefato, abordando-se as condutas pré, trans e pós-operatórias. Observando-se que é requerido dos Cirurgiões Buco-Maxilo-Faciais, plenos domínios tático, técnico e teórico, para se obter êxito no planejamento e sucesso no tratamento dessas lesões.

ABSTRACT Injuries caused by firearm projectiles in the head and neck region generally imply severe morbidity and mortality, representing an important public health problem. These injuries can be classified as: penetrating, perforating and avulsive, and the latter is the most worrisome, because they are caused by high-velocity projectiles. Low-velocity projectiles commonly cause penetrating injuries, sometimes remaining lodged in tissues, and their removal may or may not be indicated, depending on factors such as location and severity of the lesion. This paper presents the report of three clinical cases that address injuries caused by low-velocity firearm projectiles, in which they had the indication of this artifact removal, addressing the pre, trans and post-surgery procedures. And we point out that full tactical, technical and theoretical domains are required from the Oral-Maxillofacial Surgeons for being successful in the planning and success in the treatment of these lesions.